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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -GFAP (Glial Fibrillary Acidic Protein) (BSA & Azide Free)

Anti -GFAP (Glial Fibrillary Acidic Protein) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Glial fibrillary acidic protein is specific to astrocytes and ependylmal cells of the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the central nervous system.
Catalog #G2032-25X
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native and denatured): 2ug/mg protein lysate (Use Protein G).
Western Blot: 1-2ug/ml for 2 hrs at RT
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT. No special pretreatment is required for staining of formalin-fixed tissues.
Optimal dilutions to be determined by the researcher.
Source Ascites
Positive Control Astrocytoma, IMR5 and brain cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.308
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and sodium azide. See G2032-25.
PurityPurified by Protein G affinity chromatography.
ImmunogenPorcine spinal cord. Cellular Localization: Cytoplasmic.
SpecificityRecognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Many types of neural tumors such as neuroblastomas Schwannamoas, as well as extra-CNS tumors are not labeled. Species Crossreactivity: human, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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