| Human GFAP is a 49.7kD protein (322 amino acids) expressed in astrocytes. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Various mutations of the GFAP genes in humans produce Alexander’s disease, one of the leukodystrophies. |
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| Catalog # | G2032-27H |
| Applications | Suitable for use in Immunocytochemistry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Immunocytochemistry: 1:100-1:200 |
| Immunofluorescence: 1:1000 |
| Western Blot: 1:500 |
| Immunohistochemistry: 1:100-1:200 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906: IgY, Chicken Pab Rb x Ch |
| I1906-10: IgY, Chicken (HRP) Rb x Ch |
| I1906-12: IgY, Chicken (FITC) Rb x Ch |
| I1906-14: IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
| Purity | Purified by affinity chromatography |
| Immunogen | Recombinant protein corresponding to mouse GFAP. |
| Specificity | Recognizes mouse GFAP. Species Crossreactivity: human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
