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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -GFAP (Glial Fibrillary Acidic Protein)

Anti -GFAP (Glial Fibrillary Acidic Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Catalog #G2032-28B
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: No special pretreatment required prior to staining of formalin-fixed, paraffin-embedded sections.
Optimal dilutions to be determined by the researcher.
Positive ControlIMR-5 cells, Brain or astrocytoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F186
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G2032-28BX.
PurityPurified by Protein G affinity chromatography.
ImmunogenGlial Fibrillary Acidic Protein (51-52kD). Cellular Localization: Cytoplasmic
SpecificityRecognizes human GFAP. Stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Does not stain many types of neural tumors such as neuroblastomas, Schwannomas and extra-CNS tumors. Species Crossreactivity: porcine, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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