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You are here:Home » Molecular Biology » MB-Growth Factors-GFAP » GFAP, Human (Glial Fibrillary Acidic Protein)

GFAP, Human (Glial Fibrillary Acidic Protein)


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Protein (GFAP), as a member of the cytoskeletal protein family, is the principal 8-9nm intermediate filament in mature astrocytes of the central nervous system (CNS). GFAP is a monomeric molecule with a molecular mass between 40 and 53kD and an isoelectric point between 5.7 and 5.8. GFAP is a highly brain-specific protein that is not found outside the CNS. Findings have shown that while GFAP is released into the blood very soon after traumatic brain injury (TBI) based on its severity, GFAP is not released after even multiple traumae in the absence of brain injury. In the CNS, following injury, either as a result of trauma, disease, genetic disorders, or chemical insult, astrocytes become reactive and respond in a typical manner, termed astrogliosis. Astrogliosis is characterized by the rapid synthesis of GFAP. GFAP normally increases with age and there is a wide variation in the collection and processing of human brain tissue. Thanks to its high brain specificity and early release from the CNS after TBI, GFAP might be a suitable marker for early diagnostics.
Catalog #G2032-33B
Molecular Weight ~46kD(major two bands). GFAP preparations were represented by several protein bands 43-49kD. The major two bands appear in the region of the 46kD.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~46kD
SourceHuman, normal brain
Purity~90% by 10% SDS-PAGE: Two staged tissue extraction, hydroxyapatite column puri cation
FormSupplied as a suspension in 800mM urea, 1mM sodium phosphate, 16mM sodium bicarbonate. No preservative added.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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