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You are here:Home » Antibodies » Abs to Enzymes, Aminotransferase » Anti -GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT, Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT, D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1)

Anti -GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT,
Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT,
D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP
GFAT1, glutamine:fructose-6-phosphate aminotransferase 1, is the rate-limiting enzyme of the hexosamine biosynthesis pathway (1). This enzyme catalyzes the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate and glutamate (2). The
hexosamine biosynthesis pathway generates the building blocks for protein and lipid glycosylation (2). Furthermore, studies suggest that increased activity of this pathway is a contributing factor to hyperglycemia-induced insulin resistance (1,2). GFAT1 is more active in non-insulin-dependent diabetes mellitus (NIDDM) patients (3). Transgenice mice overexpressing this enzyme in skeletal muscle and adipose tissue show an insulin resistance phenotype (4,5).
Catalog #G2032-90H
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1035
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenSynthetic peptide corresponding to the sequence around Gly100 of human GFAT1.
SpecificityRecognizes endogenous levels of total GFAT1. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=2.6.1.16


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