| Catalog # | G2033-90 |
| Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase | they inhibit the cyclase in response to beta-adrenergic stimuli |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500-1:1000 dilution detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone. |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| I1904-40D: IgG, H&L (FITC) Pab Gt xRb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2. No preservative added. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide (KNNLKDCGLF) corresponding to the C-terminal region shared by rat Gia-1 and Gia-2, coupled to KLH. |
| Specificity | Recognizes rat Gia-1 and Gia-2, Mr 40kD. Does not crossreact with Gia-3 or Goa. Species Crossreactivity: mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
