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You are here:Home » Antibodies » Antibodies-G Proteins » Anti -Gi alpha 1,2

Anti -Gi alpha 1,2


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Catalog #G2033-90
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase they inhibit the cyclase in response to beta-adrenergic stimuli
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000 dilution detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone.
Optimal dilution determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
I1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (KNNLKDCGLF) corresponding to the C-terminal region shared by rat Gia-1 and Gia-2, coupled to KLH.
SpecificityRecognizes rat Gia-1 and Gia-2, Mr 40kD. Does not crossreact with Gia-3 or Goa. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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