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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IP

Gi alpha 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-gamma activation and Ca+2 mobilization in hepatocytes1,2. G-proteins are membrane associated heterotrimeric proteins that are comprised of alpha, beta-, and lambda-subunits. The alpha-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the alpha-subunit from the beta, lambda-subunit complex. This enables the G alpha-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the alpha-subunit with the beta, lambda- subunit complex.
Catalog #	G2033-91
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000-1:2000 detected Gi alpha 3 in rat hippocampus membrane preparation. Previous lots detected Gi apha 3 in solubilized rat and mouse brain membranes and mouse 3T3/A31 cell lysates.
	Immunoprecipitation: 4ul immunoprecipitated Gi alpha 3 from a solubilized mouse brain membrane lysate.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not Determined
Form	Supplied as a liquid in PBS, pH 7.4.
Purity	Serum
Immunogen	Synthetic peptide corresponding to 10aa, KNNLKECGLY from the C-terminal region of Gi alpha 3.
Specificity	Recognizes Gi alpha 3 and Go alpha, Mr 40kD. Low crossreactivity with other G-proteins. Species Crossreactivity: human, mouse, hamster and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.