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You are here:Home » Antibodies » Antibodies-Golgi Proteins » Anti -Giantin (Macrogolgin, Golgi Complex-associated 372kD Protein, GCP372)

Anti -Giantin (Macrogolgin, Golgi Complex-associated 372kD Protein, GCP372)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP IH IC FC
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus.
Catalog #G2034-30
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot.
Other applications not tested.
Recommended DilutionsImmunocytochemistry: 1:1000
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Optimal dilution to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6D410
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenGiantin from Caco-2 cell golgi membrane fraction.
SpecificityRecognizes monkey giantin. Species Crossreactivity: mammalian and avian cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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