| Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus. |
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| Catalog # | G2034-30 |
| Applications | Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot. |
| Other applications not tested. |
| Recommended Dilutions | Immunocytochemistry: 1:1000 |
| Immunoprecipitation: 2ul |
| Immunohistochemistry: Frozen sections |
| Optimal dilution to be determined by researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6D410 |
| Host | Mouse |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Giantin from Caco-2 cell golgi membrane fraction. |
| Specificity | Recognizes monkey giantin. Species Crossreactivity: human, mouse, rat, goat, sheep, canine, porcine, mammalian and avian cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
