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GLC8,1-229aa, Recombinant (Glc8p, Protein GLC8, YM9924.03C, YMR311C)
GLC8,1-229aa, Recombinant (Glc8p, Protein GLC8, YM9924.03C, YMR311C)
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| GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p). This protein involved in glycogen metabolism and chromosome segregation, proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. Under normal conditions, Glc8p activates Glc7p, but when Glc8p is over produced, it may also inhibit Glc7p function. Glc8p is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p). | | | Catalog # | G2035-18 | | AA Sequences | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGGI LKNPLALSPE QLAQQDPETL EEFRRQVYEN TQKNAKLTSH KRNIPGLDNT KEEGEIIGTS STFLPKDTLS LKHEQDMLAK MTPEERVQWN QRNLAENEIT KKQFQDIHID EPKTPYQGAV DPHGEYYRVD DDEDEDNSDK | | KPCQVANDDI DDLSLGEPEF EIKENKQPDF ETNDENDEDS PEARHKKFEE MRKKHYDVRA IFNKKSREAL KDEDEDEDDS TTKEP | | Molecular Weight | 30.7kD (265aa) (Real molecular size on SDS-PAGE will be shift up) | | Endotoxin | 1.0 EU/ug of protein (determined by LAL method) | | Storage and Stability | For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 30.7kD | | Source | E. coli | | Purity | 95% by SDS PAGE. Recombinant yeast GLC8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris pH 8.0, 1mM DTT, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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