| Gli1 is a member of the Krupple family of zinc finger proteins. Gli1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. Gli1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated. |
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| Catalog # | G2035-50A |
| Applications | Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1ug/ml |
| Immunocytochemistry: 5-15ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-28C: IgG, H&L (HRP) Pab DkxGt |
| I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt |
| I1904-28K: IgG, H&L (HRP) Pab RbxGt |
| I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt |
| I1904-28A IgG, H&L (FITC) DkxGt |
| I1904-28E IgG, H&L, X-Adsorbed (FITC) DkxGt |
| I1904-33E IgG, H&L (FITC) DkxGt |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | E. coli-derived recombinant mouse Gli1 (aa1-237) |
| Specificity | Recognizes mouse GLi1 at ~100kD. Species Crossreactivity: human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
