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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IC
Gli1 is a member of the Krupple family of zinc finger proteins. Gli1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. Gli1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
Catalog #G2035-50A
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-28C: IgG, H&L (HRP) Pab DkxGt
I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt
I1904-28K: IgG, H&L (HRP) Pab RbxGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt
I1904-28A IgG, H&L (FITC) DkxGt
I1904-28E IgG, H&L, X-Adsorbed (FITC) DkxGt
I1904-33E IgG, H&L (FITC) DkxGt
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant mouse Gli1 (aa1-237)
SpecificityRecognizes mouse GLi1 at ~100kD. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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