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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse B IP
GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2 and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Ci (Cubitus interruptus) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development and is aberrantly activated in many types of cancers (4,5).
Catalog #G2035-50B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1kappa
Clone No8A15
HostMouse
SourceHuman
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/mlBSA, 50% glycerol, 0.02% sodium azide.
PurityAs reported
ImmunogenImmunizing mice with a recombinant human GLI1 protein.
SpecificityDetects endogenous levels of total GLI1 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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