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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IC
GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI- activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
Catalog #G2035-50C
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunocytochemistry: 10ug/ml detects GLI-1 in A172 human glioblastoma cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-47H IgG, H&L (HRP) Pab GtxRt
I1904-47N IgG, H&L, X-Adsorbed (HRP) Pab GtxRt
I1904-50T1 IgG, H&L (HRP) Pab GtxRt
I1904-50U1 IgG H&L, X-adsorbed (HRP) Pab GtxRt
I1904-74F IgG, Murine X-Adsorbed (FITC) Pab GtxRt
HybridomaRat immunized with purified, E. coli-derived recombinant human GLI-1.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8B21
HostRat
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul of sterile PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-234 of human GLI-1, expressed in E. coli (PO8151).
SpecificityRecognizes endogenous human GLI-1. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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