| GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI- activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated. |
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| Catalog # | G2035-50C |
| Applications | Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.1ug/ml |
| Immunocytochemistry: 10ug/ml detects GLI-1 in A172 human glioblastoma cells. |
| Optimal dilutions to be determined by the researcher. |
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| Recommended Secondary Antibodies: |
| I1904-47H IgG, H&L (HRP) Pab GtxRt |
| I1904-47N IgG, H&L, X-Adsorbed (HRP) Pab GtxRt |
| I1904-50T1 IgG, H&L (HRP) Pab GtxRt |
| I1904-50U1 IgG H&L, X-adsorbed (HRP) Pab GtxRt |
| I1904-74F IgG, Murine X-Adsorbed (FITC) Pab GtxRt |
| Hybridoma | Rat immunized with purified, E. coli-derived recombinant human GLI-1. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 8B21 |
| Host | Rat |
| Source | Human |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul of sterile PBS, 0.02% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant corresponding to aa1-234 of human GLI-1, expressed in E. coli (PO8151). |
| Specificity | Recognizes endogenous human GLI-1. Species crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
