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You are here:Home » Antibodies » Antibodies-Transcription Factors, Zinc (Ring) Finger » Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IC
GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI- activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
Catalog #G2035-50C
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunocytochemistry: 10ug/ml detects GLI-1 in A172 human glioblastoma cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-47H IgG, H&L (HRP) Pab GtxRt
I1904-47N IgG, H&L, X-Adsorbed (HRP) Pab GtxRt
I1904-50T1 IgG, H&L (HRP) Pab GtxRt
I1904-50U1 IgG H&L, X-adsorbed (HRP) Pab GtxRt
I1904-74F IgG, Murine X-Adsorbed (FITC) Pab GtxRt
HybridomaRat immunized with purified, E. coli-derived recombinant human GLI-1.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No8B21
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul of sterile PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-234 of human GLI-1, expressed in E. coli (PO8151).
SpecificityRecognizes endogenous human GLI-1. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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