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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GLI2 (GLI-Kruppel Family Member 2, GLI-Kruppel Family Member GLI2, GLI Family Zinc Finger 2, HPE9, Tax Helper Protein, THP, THP1, THP2, Zinc Finger Protein GLI2)

Anti -GLI2 (GLI-Kruppel Family Member 2, GLI-Kruppel Family Member GLI2, GLI Family Zinc
Finger 2, HPE9, Tax Helper Protein, THP, THP1, THP2, Zinc Finger Protein GLI2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
GLI2, also known as GLI-Kruppel family member 2, a 133.3kDa protein belonging to the C2H2-type zinc finger protein subclass of the Gli family of transcription factors. GLI2 and the related GLI3 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI2 being primarily a transcriptional activator while GLI3 is a transcriptional repressor. Studies indicate that GLI2 plays a role in embryogenesis. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.
Catalog #G2035-53D
ApplicationsSuitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 5ug/ml. Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 0.01M sodium citrate buffer, pH 6 is recommended.
ELISA: 1:15,000-1:60,000
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Positive Control for ImmunohistochemistryMouse brain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to an amino acid sequence from an internal region of mouse GLI2.
SpecificityRecognizes mouse GLI2. Species Crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesOncogene GLI2, Tax Helper Protein 1, Tax Helper Protein 2, Tax Responsive Element 2 Holding Protein, Tax Responsive Element-25 bp Sequence Binding Protein


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