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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Gliadin (Celiac Disease)

Anti -Gliadin (Celiac Disease)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
Catalog #G2035-86
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: Reacts with deamidated peptide (KLQPFPQPELPYPQPQ) and non-deamidated peptide (KLQPFPQPQLPYPQPQ) when coated directed in an immunoplate
Western Blot: 1:4000
Optimal dilutions to be determined by the researcher.
Positive ControlsBarley, rye and wheat
Negative ControlOats
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No6F113
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide
PurityPurified by Protein A affinity chromatography
ImmunogenGliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ). Epitope: Located in the central region of the Lys57-Glu65-[alpha-gliadin (58-73)] peptide.
SpecificityRecognizes Gliadin. Reacts equally strong with both the deamidated peptide (KLQPFPQPELPYPQPQ) and the non-deamidated peptide (KLQPFPQPQLPYPQPQ) when coated directly in an immunoplate. Reactivity: wheat, barley and rye.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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