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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Gliadin (Celiac Disease)

Anti -Gliadin (Celiac Disease)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
Catalog #G2035-86
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA: 1:20,000 using deamidated peptide (KLQPFPQPELPYPQPQ) coated at 0.1ug/ml.
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 2-10% fetal calf serum
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No6F113
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenGliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) adsorbed onto aluminum hydroxide gel.
SpecificityRecognizes Gliadin. Reacts equally strong with both the deamidated peptide (KLQPFPQPELPYPQPQ) and the non-deamidated peptide (KLQPFPQPQLPYPQPQ) when coated directly in an immunoplate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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