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You are here:Home » Molecular Biology » Growth Factors-GDNF » Glial Derived Neurotrophic Factor (GDNF) Recombinant, Human

Glial Derived Neurotrophic Factor (GDNF) Recombinant, Human

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Specifications

This factor was found initially in conditioned medium of the rat B49 glial cell line. GDNF is a disulfide-bonded homodimeric glycosylated protein of 134 amino acids. The proteins contain seven conserved cysteine residues in the same relative spacing found in all members of the TGF-beta superfamily of proteins. The human GDNF gene has been mapped to chromosome 5p13.1-p13.3. Related factors are Neurturin, Persephin, and Artemin. Collectively they are being referred to as GDNF family ligands.
Catalog #G2036-02B
Endotoxin <0.01ng/ug or <0.1EU/ug as per LAL
AA SequenceMSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Biological Activity Glial-Derived Neurotrophic Factor is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent dopamin uptake in rat mesencephalic cultures was found to be 5-10ng/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight30.4kD
SourceRecombinant, human, E. coli
Purity98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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