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You are here:Home » Molecular Biology » Growth Factors-GDNF » Glial Derived Neurotrophic Factor, Recombinant, Human (GDNF)

Glial Derived Neurotrophic Factor, Recombinant, Human (GDNF)

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Specifications

Human GDNF (Glial-Derived Neurotrophic Factor) is a 30.1kD protein that specifically promotes dopamine uptake and survival of midbrain neurons. GDNF is a disulphide-linked homodimer, formed by two identical 134aa subunits.
Catalog #G2036-18
SourceRecombinant corresponding to human GDNF expressed in E. coli.
Biological ActivityThe ED50 as determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures was found to be in the range of 4-8ng/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~30.1kD
SourceE.coli
Purity~98% (SDS-PAGE and HPLC). Endotoxin: <0.1EU/ug
ConcentrationNot determined
FormSupplied as a lyophilized powder from 10mM sodium citrate, 150mM sodium chloride, pH 4. Reconstitute in sterile ddH2O to 0.1-1.0mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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