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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum IH IF

Glucagon is produced by the alpha-cells of the pancreatic islets. It occurs also in endocrine cells of the gut and in neurons in the brain.
Catalog #	G2040-01
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (PAP): 1:1000-1:2000; requires overnight incubation at 4ºC.
	Note: Suitable for frozen and paraffin-embedded sections. No proteolytic treatment is required.
	Optimal dilutions to be determined by the researcher.
Positive Control	Bouin-fixed paraffin sections of pancreas
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Porcine
Concentration	Not Determined
Form	Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile ddH2O. Dilute further in 0.1M PBS, 1% BSA, 0.1% sodium azide.
Purity	Serum
Immunogen	Highly purified porcine pancreatic glucagon. Cellular Localization: Cytoplasmic
Specificity	Recognizes porcine Glucagon. Can be used for the detection of pancreatic endocrine tumors. Absorption with 10-100ul glucagon per ml diluted antiserum abolishes staining.
Species Crossreactivity	Human and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.