| GLP1, a processed active peptide of 30aa (chr 2q36-q37) is a potent insulin secretagogue, and plays a major role in the enteroinsular axis, accounting for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously. |
|
| Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulate intestinal growth and up regulate villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration. |
|
| Catalog # | G2040-35C |
| Applications | Suitable for use in Electron Microscopy, Dot Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (Paraffin): 1:80-1:8000 |
| Electron Microscopy: 1:600 |
| Immunocytochemistry: 1:10,000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | GLP-1/18 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute in 100ul sterile ddH2O. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa7-36 of human GLP-1 conjugated to KLH. |
| Specificity | Recognizes human GLP-1 (aa7-36) and GLP-1(aa1-36). Does not cross react with GLP2, GRPP, PYY, VIP, PHI, GIP, Glucagon or Oxyntomodulin. Species Crossreactivity: rat and porcine. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
