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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Glucose-Regulated Protein 94

Anti -Glucose-Regulated Protein 94

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH
Grpís (Glucose-Regulated Proteins) are a class of proteins synthesized in cells in response to glucose starvation. Glucose-regulated protein 94 (grp94) or tumor rejection antigen (gp96) shows a high degree of sequence homology with the heat shock protein 90 (hsp90). Grp94 has been briefly studied as a prognostic factor in breast cancer.
Catalog #G3057
ApplicationsSuitable for use in Immunoprecipitation (native only), Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: Use Protein G; 2ug/mg protein lysate
Western Blot 1-2ug/ml for 2 hrs at RT.
Immunohistochemistry (frozen & formalin/paraffin) 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive controlSKBR-3 cells. Tonsil
Cellular LocalizationCytoplasmic and nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.N.311
HostRat
SourceChicken
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G3057X.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified glucose regulated protein 94 (grp94) from chicken oviducts
SpecificityRecognizes chicken glucose-regulated protein 94 (grp94) at 94kD. Species Crossreactivity: Human, monkey, equine, bovine, sheep, porcine, canine, rabbit, rat, mouse, hamster, guinea pig and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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