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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Glucose Regulated Protein 78 (Hspa5, Heat Shock Protein 5, 78kD, 78kD Glucose-Regulated Protein precursor, AL022860, AU019543, Bip, BiP, D2Wsu141e, D2Wsu17e, Grp78, GRP 78, Heat shock 70kD protein 5, Heat Shock 70kD Protein 5, Hsce70, Immunoglobulin heavy

Anti -Glucose Regulated Protein 78 (Hspa5, Heat Shock Protein 5, 78kD, 78kD
Glucose-Regulated Protein precursor, AL022860, AU019543, Bip, BiP, D2Wsu141e, D2Wsu17e,
Grp78, GRP 78, Heat shock 70kD protein 5, Heat Shock 70kD Protein 5, Hsce70,
Immunoglobulin heavy

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B
The immunoglobulin heavy chain binding protein BiP (Binding Protein, [1]) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as “molecular chaperones,” which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (mu+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7
Catalog #G3057-20G
ApplicationsSuitable for use in ELISA, Western Blot and Studies of immunoglobulin assembly and secretion. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K23
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
SpecificityRecognizes mouse BiP (Immunoglobulin heavy chain binding protein, Mr 78kD). Recognizes a conserved epitope localized within the region of aa497 to 581 of BiP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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