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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Glucose Regulated Protein 78 (Grp78, BiP, KDEL)

Anti -Glucose Regulated Protein 78 (Grp78, BiP, KDEL)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
The 78kD glucose regulated protein/BiP (GRP78) belongs to the family of ~70kD heat shock proteins (HSP70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen.
Catalog #G3057-23B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a lyophilized powder with 0.05% sodium azide. Reconstitute with 100ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 643-654 (TGEEDTSEKDEL) of rat GRP78 (glucose regulated protein 78kD).
SpecificityRecognizes rat GRP78. Does not detect GRP78 from human cell lines or COS cells. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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