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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Glucose Regulated Protein 94 (Grp94, gp96)

Anti -Glucose Regulated Protein 94 (Grp94, gp96)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP FC
Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion
(1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2) Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90 (3). Both Hsp90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
Catalog #G3057-40B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml was sufficient for detection of Grp94 in 20ug of HeLa lysate; Goat anti-rat IgG:HRP as the secondary antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7L6
HostRat
SourceChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified Grp94 isolated from chicken oviducts
SpecificityDetects a 98kD protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74 or GrpE from E. coli. Species Crossreactivity: Human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, equine, monkey, porcine, rabbit, sheep, Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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