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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Glucose Regulated Protein 94 (Grp94, gp96)

Anti -Glucose Regulated Protein 94 (Grp94, gp96)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC
Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which belongs to the Hsp90 family of molecular chaperones. Grp94 possesses the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal thought to lead to the retention of proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation. Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol. . Recent data has identified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).
Catalog #G3057-42
ApplicationsSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:80
Optimal dilutions to be determined by researcher.
Positive ControlsMouse Brain Tissue Extract or Rat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.85mg/ml
FormSupplied as a liquid in borate buffered saline, pH 8.2 (25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt))
PurityPurified by Protein A affinity chromatography.
ImmunogenA 16 residue synthetic peptide (C)EEEEETEKESTEKDEL based on
the mouse Grp94 (residues 787-802) with the cysteine (C) residue added and the peptide coupled to KLH.
SpecificityDetects a ~94kD protein, corresponding to the apparent molecular mass of Grp94 on SDS-PAGE immunoblots. Species Crossreactivity: human, mouse, rat, bovine (weakly) and canine. Antibody specificity is confirmed by peptide inhibition studies.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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