| The tumor rejection antigen (gp96), also known as glucose-regulated protein 94 (GRP94), is a member of the heat shock protein (HSP) family. The gp96 protein is up-regulated by stress conditions such as glucose starvation and heat shock, and its function is regulated through a ligand-dependent conversion from an active to an inactive conformation. gp96 is involved in both innate and adaptive immunity. A study has shown that a B-cell line deficient in gp96 is unresponsive to microbial stimuli because toll-like receptors (TLRs) are retained intracellularly. However, normal surface levels of TLRs were restored when these cells were transfected with a cDNA encoding gp96. The tumor rejection antigen can activate the release of nitric oxide by antigen presenting cells (APCs) and it also has a relevant role in the regulation of immune responses to pathological cell death. |
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| Catalog # | G3057-43 |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | ELISA: 0.1-1ug/ml |
| Western Blot: 1-3ug/ml. Identifies a single band at ~96kD |
| Immunoprecipitation: 1-5ug/ml |
| Immunohistochemistry: 0.5-1ug/ml Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining for formalin-fixed, paraffin-embedded (FFPE) tissues. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | 0..25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the N-terminal region of the mouse and human gp96 protein. Species Sequence Homology |
| Specificity | Recognizes mouse, rat and human gp96 proteins. Reactivity has been confirmed with mouse and rat brain homogenates by Western Blot and immunoprecipitation, and with formalin-fixed, paraffin-embedded (FFPE) human tonsil tissue by immunohistochemistry. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
