| GrpE is an acidic 24 kD protein encoded by a monocistronic Escherichia coli gene (located at 56 min.) which is under the control of both s32 and s70 transcription factors. The GrpE protein functions as a co-chaperone in assisting the DnaK (Hsp70) protein to effectively carry out DnaK-dependent chaperone activity (i.e., protein folding, protein transport, disaggregation of heat inactivated proteins, activation of mutant proteins). DnaK and DnaJ assist protein folding by |
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| stabilizing an intermediate conformation; where as the GrpE protein, in an ATP-dependent reaction, releases partially folded protein from the DnaK/J complex and transfers this immature protein to the GroEL/ES chaperone complex to finish the protein folding reaction2. GrpE co-chaperone protein assists the DnaK chaperone during two crucial steps: binding to protein substrate and releasing DnaK chaperone from the complex with substrate. In the first case, the |
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| presence of GrpE protein releases those substrates not tagged by the DnaJ protein from DnaK complexes. In this case, GrpE protein works as a discrimination factor. In the second step, GrpE and DnaJ proteins jointly accelerate DnaK’s ATPase activity up to 50 fold. In this reaction, GrpE |
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| protein is responsible for the release of ADP from DnaK, thus recycling DnaK protein to the DnaK-ATP form. Eukaryotic GrpE homologues in yeast suggest similar co-chaperone action in eukaryotic cells. |
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| Catalog # | G3057-63 |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot (Colorimetric): 1:1000 |
| Optimal dilutions to be determined by researcher. |
| Positive Controls | G3057-60: Glucose Regulated Protein E, Recombinant (GrpE) |
| Stprage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | E. coli |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in TBS, pH 7.5, 0.1% sodium azide, 50% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Purified E. coli GrpE Protein |
| Specificity | Recognizes E. coli GrpE. Detects a 24kD protein, corresponding to the apparent molecular mass of GrpE on SDS-PAGE immunoblots, in samples from E. coli. Does not react with proteins from human, mouse, rat nor yeast lysates. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
