| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. |
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| GLUT 1 is a facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. It has a very broad substrate specificity and can transport a wide range of aldoses including pentoses and hexoses. |
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| Catalog # | G3900-01A |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 1:10,000-1:50,000; Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Western Blot: 1:1000-1:5000; Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1). Not recommended to heat the samples prior to loading to prevent Glut protein aggregation. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | G3900-01D: GLUT 1, Mouse, Control Peptide (Glucose Transporter 1) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, 0.05% sodium azide. |
| Purity | Serum |
| Immunogen | 12aa peptide sequence near the C-terminal, cytoplasmic domain of mouse brain glucose transporter-1 (Glut-1) (KLH). Cellular Localization: Cell membrane; Localizes primarily at the cell surface. |
| Specificity | Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins. Species Sequence Homology: 100% conserved in human, rat, rabbit, bovine and porcine; 90% conserved in chicken. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
