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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family). Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, MW 40-60kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Catalog #	G3900-01C
	Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier. It is ubiquitously expressed and transport glucose in most cells.
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-10ug/ml (ECL). Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1). Not recommended to heat the samples prior to loading to prevent Glut protein aggregation.
	ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
	Optimal dilutions to be determined by the researcher.
Control Peptide	G3900-01D
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as liquid in PBS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide consisting of 12aa sequence near the C-terminus of mouse/rat brain glucose transporter-1 (Glut-1) (KLH coupled).
Specificity	Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins. Species sequence homology: human, rat, rabbit, bovine and porcine (100%); chicken (90%).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.