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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -GLUT1 (Glucose Transporter 1, Solute Carrier Family 2, Facilitated Glucose Transporter Member 1, Glucose Transporter Type 1, Erythrocyte/Brain, GLUT-1, HepG2 Glucose Transporter, SLC2A1)

Anti -GLUT1 (Glucose Transporter 1, Solute Carrier Family 2, Facilitated Glucose
Transporter Member 1, Glucose Transporter Type 1, Erythrocyte/Brain, GLUT-1, HepG2 Glucose
Transporter, SLC2A1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
Catalog #G3900-01M
ApplicationsSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-5ug/ml using chemiluminescence techniques. It is recommended not to heat the samples prior to loading to prevent gluts aggregation.
Flow Cytometry: ~10ug/ml (10e6 cells); Perform the antibody binding in small volume of ~200ul.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman liver tumor cells (HepG2)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11C100
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a lyophillized powder in PBS, pH 7.5. Reconstitute in 100ul of sterile PBS.
PurityPurified by Protein A/G affinity chromatography
ImmunogenRecombinant protein corresponding to human GLUT1.  Epitope: Extracellular domain. 
SpecificityRecognizes human GLUT1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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