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Abs to Glucose Transporters
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Anti -GLUT1 (Glucose Transporter 1, Solute Carrier Family 2, Facilitated Glucose Transporter Member 1, Glucose Transporter Type 1, Erythrocyte/Brain, GLUT-1, HepG2 Glucose Transporter, SLC2A1)
Anti -GLUT1 (Glucose Transporter 1, Solute Carrier Family 2, Facilitated Glucose
Transporter Member 1, Glucose Transporter Type 1, Erythrocyte/Brain, GLUT-1, HepG2 Glucose
Transporter, SLC2A1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B FC |
|
| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. | | | Catalog # | G3900-01M | | Applications | Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-5ug/ml using chemiluminescence techniques. It is recommended not to heat the samples prior to loading to prevent gluts aggregation. | | Flow Cytometry: ~10ug/ml (10e6 cells); Perform the antibody binding in small volume of ~200ul. | | Optimal dilutions to be determined by the researcher. | | Western Blot Positive Control | Human liver tumor cells (HepG2) | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 11C100 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with 100ul sterile PBS. | | Purity | Purified by Protein A/G affinity chromatography | | Immunogen | Recombinant protein corresponding to human GLUT1, Extracellular domain. Cellular Localization | | Specificity | Recognizes human GLUT1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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