| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins ( Glut-1 to Glut-7 , MW 40-60kD) and Sodium Glucose Co-Transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. |
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| Catalog # | G3900-05A |
| Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons. Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7 is expressed in liver and other gluconeogenic tissues and mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa, , monkey 621aa; ~ 50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue, and small intestine. |
| Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000. Identifies a major band at about 42-45kD |
| ELISA: 1:10,000-50,000. Control Peptide (G3900-05E) can be used to coat ELISA plates at 1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | ELISA: G3900-05E, GLUT 1, Control Peptide, Human |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol. |
| Purity | Serum |
| Immunogen | Synthetic peptide 13aa sequence near the C-terminus of human Glut-1 (KLH coupled). |
| Specificity | Recognizes GLUT 1. Does recognize Hep G2 type transporter and Glut-1 from Caco-2 cells. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
