| Most mammalian cells transport glucose through a family of |
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| membrane proteins known as glucose transporters. Molecular |
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| cloning of these glucose transporters has identified a family of |
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| closely related genes that encodes at least 7 proteins (Glut-1 to |
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| Glut-13, Mol. Wt. 40-80kD) and sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue- specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. |
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| Catalog # | G3900-05K |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1-10ug/ml using ECL. Identifies a major band at about 42-45kD from purified rat brain membranes. |
| ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | G3900-05L: GLUT 1 (Glucose Transporter 1) Control Peptide |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic peptide correesponding to 15aa near the C-terminus, cytoplasmic domain of human Glut-1 (KLH). Species Sequence Homology: porcine, bovine, rabbit, mouse and rat; 100%. |
| Specificity | Recognizes human GLUT 1 (Glucose Transporter 1). Antibodies made to this sequence from this region have detected Glut-1 from human (RBC) and rabbit brain and mouse 3T3 L1 fibroblasts and rat brain adipocytes. It also recognizes the Hep G2 type transporter and Glut-1 from Caco-2 cells (3). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
