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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.
Catalog #	G3900-12
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
	Western Blot: 5ug/ml, adipose and muscle tissue
	Immunohistochemistry: 5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.1, 0.1% sodium azide.
Purity	Purified by affinity chromatography on a Sepharose 4B CNBr linked peptide column.
Immunogen	Peptide corresponding to 12aa, LFHPLGADSQV, of the C-terminus of human GLUT-1, conjugated to KLH.
Specificity	Recognizes human glucose transporter 1 from muscle and adipose tissue. Does not crossreact with GLUT-4. The MW of the glucose transporter precipitated by the serum is ~46kD.
Species Crossreactivity	Rat and mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.