| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, 40-80kD) and sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. |
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| Catalog # | G3900-15D |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Western Blot: 1-10ug/ml using Chemiluminescence technique. |
| Immunohistochemistry: 2-10ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | G3900-15F: GLUT 2, Rat (Glucose Transporter, Insulin Regulatable) |
| Recommended Tissues | Liver, intestine, kidney, and beta-pancreatic islet cells. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic peptide corresponding to 16aa near the C-terminal, cytoplasmic domain of rat Glut-2 (KLH). Subcellular Location: multi-pass membrane protein. |
| Specificity | Recognizes rat Glut-2. Species Sequence Homology: mouse and human-93%, ovine-84%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
