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Abs to Glucose Transporters
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Anti -GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)
Anti -GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose
Transporter Type 2, Liver, GLUT-2, SLC2A2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
B FC |
|
| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. | | | Catalog # | G3900-15Q | | Applications | Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-5ug/ml (ECL). Do not to heat the samples prior to loading to prevent gluts aggregation. Mouse tissues or mouse insulinoma beta TC-6 cells can be used as positive control. | | Flow Cytometry: ~10ug/ml (10e5 cells) Perform the antibody binding in small volume of ~200ul. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 11C102 | | Host | Rat | | Source | Mouse | | Concentration | ~1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS. Reconstitute with 100ul of sterile PBS. | | Purity | Purified by Protein A/G affinity chromatography | | Immunogen | Recombinant corresponding to mouse GLUT2. Epitope: Extracellular domain | | Specificity | Recognizes mouse GLUT2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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