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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

Anti -GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose
Transporter Type 2, Liver, GLUT-2, SLC2A2)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH FC
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis.
Catalog #G3900-15Q
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-5ug/ml (ECL). Do not to heat the samples prior to loading to prevent gluts aggregation. Mouse tissues or mouse insulinoma beta TC-6 cells can be used as positive control.
Immunohistochemistry: 5-20ug/ml in 4% paraformaldehyde, 0.15% picric acid for 20 minutes and then permeabilized with 0.1% Triton X-100.
Flow Cytometry: ~10ug/ml (10e5 cells) Perform the antibody binding in small volume of ~200ul.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11C102
HostRat
SourceMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS. Reconstitute with 100ul of sterile PBS.
PurityPurified by Protein A/G affinity chromatography
ImmunogenRecombinant protein corresponding to mouse GLUT2. Epitope: Extracellular domain
SpecificityRecognizes mouse GLUT2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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