| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. |
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| GLUT isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT 1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. GLUT 2 provides glucose to the liver and pancreatic cells. GLUT 3 is the main transporter in neurons, whereas GLUT 4 is primarily expressed in muscle and adipose tissue and regulated by insulin. |
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| Catalog # | G3900-24A |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000 (ECL); Detects a major band at ~45kD. |
| ELISA: 1:10,000-50,000; Coat plates at 1ug/ml with Control Peptide. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | G3900-24D: GLUT 3, Human (Glucose Transporter) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, 0.05% sodium azide and 40% glycerol. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to 12aa sequence in the C-terminus of human glucose transporter-3 (hGLUT 3) (KLH coupled). |
| Specificity | Recognizes human GLUT 3 (Glucose Transporter). |
| Species Crossreactivity | Does not recognize mouse/rat GLUT 3. For mouse and rat samples use G3900-25 or G3900-26. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
