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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -GLUT 3 (Glucose Transporter)

Anti -GLUT 3 (Glucose Transporter)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa, , monkey 621aa;~ 50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue and small intestine. Glut-13 or proton myo-inositol transporter (HMIT; human 629aa, rat 618aa, ~75-90kD/67kD protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol. Rat HMIT is ~35% identical to rat GlutX1.
Catalog #G3900-24C
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml (ECL). Detects a major band at ~45kD.
ELISA: Coat plates at 1ug/ml with Control Peptide.
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.
Control PeptideG3900-24D: GLUT 3, Human (Glucose Transporter)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromatography.Product Type: Pab
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 12aa sequence in the C-terminus of human glucose transporter-3 (hGlut-3) (KLH).
SpecificityRecognizes human GLUT 3 (Glucose Transporter). Species Crossreactivity: Does not recognize mouse/rat Glut-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
ImmunogenSynthetic peptide corresponding to 12aa sequence in the C-terminus of human glucose transporter-3 (hGlut-3) (KLH).
SpecificityRecognizes human GLUT 3 (Glucose Transporter). Species Crossreactivity: Does not recognize mouse/rat Glut-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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