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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, Mol. Wt. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoform differ in their tissue expression, substrate specificity and kinetic characteristics.
Catalog #	G3900-24G
Applications	Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 1-10ug/ml using ECL.
	ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
	Optimal dilutions to be determined by the researcher.
Control Peptide	G3900-24H: GLUT 3, Human, Control Peptide (Glucose Transporter)
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to 13aa sequence from human GLUT 3. Epitope: ~N-terminus, Extracellular domain
Specificity	Recognizes human GLUT 3. No significant homology is detected with other species. Species sequence homology: 92% with GT14 (duplicon of GT3) short and long form.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.