Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -GLUT 3 (Glucose Transporter)

Anti -GLUT 3 (Glucose Transporter)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family). Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, MW 40-60 kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
Catalog #G3900-26
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL. Major band at ~45kD.
ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml (antigen)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Store long-term at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 12 aa C-terminal peptide sequence near the C-terminus of mouse glucose transporter-3 (Glut-3). A cysteine has been added at the NH2 terminus for coupling to KLH.
SpecificityRecognizes Glut-3. The mouse Glut-3 peptide sequence used for antibody production is 100% identical in rat. This antibody is specific for mouse and rat. Does not recognize human Glut-3 because of significant interspecies differences in C-terminal Glut-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links