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Anti -GLUT 4 (Glucose Transporter 4, Glucose Transporter Type 4 Insulin-responsive, GLUT4, GLUT-4, Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 4, SLC2A4)
Anti -GLUT 4 (Glucose Transporter 4, Glucose Transporter Type 4 Insulin-responsive, GLUT4,
GLUT-4, Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 4, SLC2A4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 (mouse/human 509aa) is primarily expressed in muscle and adipose tissue and regulated by insulin. | | | Catalog # | G3900-50J | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-10ug/ml using ECL. | | ELISA: 1:1000-1:10,000. Control peptide (Cat #G3900-50J) can be used to coat plates at 1ug/ml. | | Immunohistochemistry: 2-10ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder in PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS. | | Purity | Purified by affinity chromatography. | | Immunogen | Synthetic peptide corresponding to 13aa near the N-terminal, extracellular domain of human Glut-4, conjugated to KLH. Species Sequence Homology: bovine; 92%, porcine; 84% and mouse and rat; 83% | | Specificity | Recognizes human Glut-4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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