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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
Catalog #	G3900-60D
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-10ug/ml (ECL.)
	ELISA: 0.5-1ug/ml. Coat ELISA plates with 1ug/ml control peptide.
	Optimal dilutions to be determined by the researcher.
Control Peptide	G3900-60F
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as liquid in PBS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A cytoplasmic 12aa C-terminal peptide sequence specific for the mouse Glut-4 (KLH coupled).
Specificity	Recognizes mouse Glut-4. Does not recognize other glucose transporters. Species Sequence Homology
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.