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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH IF

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-60kD) and Sodium glucose co-trasporter-1 (SGLT-1, 662aa, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Catalog #	G3900-65D
Applications	Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1-10ug/ml using ECL. The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that is slightly larger than that reported in human GLUT 5 (50-55kD). In rat kidney, a protein of ~42kD was detected.
	ELISA: 1:1000- 1:10000. Use G3900-65G control peptide to coat ELISA plates at 1ug/ml.
	Immunohistochemistry: See reference (2).
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~1mg/ml
Form	Supplied as liquid in PBS, pH 7.4, 0.1% BSA.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresonding to 13aa near the C-terminal, cytoplasmic domain of rat Glut-5. (KLH).
Specificity	Recognizes rat Glut-5. Species sequence homology
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.