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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -GLUT 7 (Glucose Transporter)

Anti -GLUT 7 (Glucose Transporter)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family). Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, Mol. Wt. 40-60 kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Catalog #G3900-80B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:1-10ug/ml using ECL. Detects a major band at about 52kD in various tissues (2).
ELISA: 0.5-1ug/ml, Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control PeptideG3900-80C GLUT 7, Rat Control Peptide.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration1mg/ml
FormSupplied as liquid in 100mM Tris, pH 7.4 and 0.2% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 14 AA peptide sequence near the C-terminus of rat Glucose Transporter 7 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.
SpecificityRecognizes rat GLUT 7. Species sequence homology
glucoronosyltransferase isoenzymes (see refs. 1). The KK-K-- has been considered motif for the retention of transmembrane proteins (3) and is not found in Gluts.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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