| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. |
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| Catalog # | G3900-95A |
| Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000 using ECL. |
| ELISA: 1:10,000-50,000; Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | G3900-95 GLUT 10, Control Peptide, Human (Glucose Transporter) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol. |
| Purity | Serum |
| Immunogen | A 16-AA peptide sequence near the cytoplasmic, C-terminus of Human Glut-11 (1) was coupled to KLH |
| Specificity | Recognizes human GLUT10. Human peptide sequence has no significant sequence homology with other gluts. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
