| Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. |
|
| Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudoegene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496 aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. |
|
| Catalog # | G3900-95D |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-10ug/ml using ECL. |
| ELISA: 0.5-1ug/ml. Control peptide can be used at 1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | G3900-95 |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.5 and 0.05% sodium azide, 40% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A 16-AA peptide sequence near the cytoplasmic, C-terminus of Human Glut-11 (1) was coupled to KLH |
| Specificity | Recognizes human GLUT 10 (. Human peptide sequence is 5% conserved in mouse Glut-10. No significant sequence homology with other gluts. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
