| Catalog # | G3990-05 |
| Applications | Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1:1000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Specificity | Using a conjugate D-Glutamate-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: |
| Compounds | Cross-reactivity ratio (a): |
| D-Glutamate-G-(Pc) 1 |
| L-Glutamate-G-(Pc) >1:50,000 |
| D-Glutamine-G-(Pc) >1:50,000 |
| L-Glutamine-G-(Pc) >1:50,000 |
| L-Aspartate-G-(Pc) >1:50,000 |
| D-Aspartate-G-(Pc) >1:50,000 |
| GABA-G-(Pc) >1:50,000 |
| Taurine-G-(Pc) >1:50,000 |
| D-Cysteine-G-(Pc) >1:50,000 D-Methionine-G-(Pc) >1:50,000 |
| D-Tryptophan-G-(Pc) >1:50,000 |
| D-T yrosine-G-(Pc) >1:50,000 |
| (a) | Glutamate-G-(Pc) concentration/other conjugated amino acid concentration at half displacement; G=Glutaraldehyde, GABA=gamma-aminobutyric acid |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid. No preservative added. |
| Purity | Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration. |
| Immunogen | Synthetic D-Glutamic acid (BSA) |
| Specificity | Recognizes protein-conjugated D-Glutamate. See additional crossreactivity data. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
