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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -Glutamate Decarboxylase 65 (GAD65)

Anti -Glutamate Decarboxylase 65 (GAD65)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Gamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is an amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).
Catalog #G4000-01C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10 ug/ml using Chemiluminescence technique
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide G4000-01A: Glutamate Decarboxylase 65, Rat, Control Peptide (GAD65)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H23
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified by affinity chromatography
ImmunogenAn 18aa peptide sequence near the N-terminus of rat GAD65. Species Sequence Homology
SpecificityRecognizes rat Glutamate Decarboxylase 65 (GAD65).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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