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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -Glutamate Decarboxylase 65 (GAD65)

Anti -Glutamate Decarboxylase 65 (GAD65)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP
Gamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is am amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).
Catalog #G4000-01D
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000- 1:5000. ~65kD band in rat brain.
ELISA: 1:10,000-1:100,000 using 50-100ng UCP2 control peptide/well.
Immunoprecipitation: 10ul.
Optimal dilutions to be determined by the researcher.
Control Peptide G4000-01A: Glutamate Decarboxylase 65, Rat, Control Peptide (GAD65)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H23
HostMouse
SourceRat
ConcentrationNot determined.
FormSupplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
PurityAscites.
ImmunogenAn 18aa peptide sequence near the N-terminus of rat GAD65.
SpecificityRecognizes rat Glutamate Decarboxylase 65 (GAD65). Species Seqeunce Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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