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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -GAD65 (Glutamate Decarboxylase 2, 65kD Glutamic Acid Decarboxylase, GAD-65, Glutamate Decarboxylase 65kD Isoform, GAD2)

Anti -GAD65 (Glutamate Decarboxylase 2, 65kD Glutamic Acid Decarboxylase, GAD-65,
Glutamate Decarboxylase 65kD Isoform, GAD2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Adults express two forms of glutamate decarboxylase (GAD), GAD65 and GAD67, which are encoded by different independently regulated genes. Both are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve endings; while GAD67 is more widely distributed throughout the cell. GAD65 preferentially synthesizes GABA for vesicular release and GAD67 preferentially synthesizes cytoplasmic GABA. GAD65 is predominantly expressed in pancreatic b cells and auto-antibodies to GAD65 are strongly associated with Insulin dependent diabetes mellitus (IMDM).
Catalog #G4000-01M
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlPancreas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of human GAD65.
SpecificityRecognizes human GAD65. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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