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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -GAD1 (Glutamate Decarboxylase 1, FLJ45882, GAD, SCP)

Anti -GAD1 (Glutamate Decarboxylase 1, FLJ45882, GAD, SCP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E
Catalog #G4000-05A
ApplicationsSuitable for use in ELISA and . Other applications not tested.
Recommended DilutionPeptide ELISA: 1:8,000.
Western Blot: 0.3-1ug/ml. Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See G4000-05A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide from the C-terminus of aa-PDSPQRREKLHK.
SpecificityRecognizes human GAD1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP


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