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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -GAD1 (Glutamate Decarboxylase 1, 67kD Glutamic Acid Decarboxylase, GAD-67, Glutamate Decarboxylase 67kD Isoform, GAD, GAD67)

Anti -GAD1 (Glutamate Decarboxylase 1, 67kD Glutamic Acid Decarboxylase, GAD-67, Glutamate
Decarboxylase 67kD Isoform, GAD, GAD67)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Glutamic acid decarboxylase (GAD) catalyzes the conversion of L-glutamic acid to the inhibitory eurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, re-encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000 polypeptide, with 85aa residues, whereas human GAD67 encodes a Mr 67,000 polypeptide, with 594aa residues. GAD67 gene consists of 16 exons, spread over more than 45kb of genomic DNA. The AD67 gene contains an additional exon (exon 0) that together with part of exon 1, specifies the 5' ntranslated region of GAD67 mRNA. Human GAD67 shows 65% identity to GAD65 and is located in q31. GAD67 may play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to ead to pyridoxine dependency with seizures.
Catalog #G4000-05B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000
Western Blot: 1-3ug/ml, observed in mouse brain lysates on ~70kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QRTNSLEEKSR, from mouse GAD1, at the internal region of the protein (NP_038473.2, NP_000808.2).
SpecificityRecognizes mouse GAD1. Species sequence homology: Bovine, canine, human, porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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