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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -Glutamate Decarboxylase 65 (GAD65)

Anti -Glutamate Decarboxylase 65 (GAD65)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Catalog #G4000-06
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining.
Optimal dilutions to be determined by the researcher.
Cellular LocalizationCytoplasmic
Positive ControlPancreas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS with sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminus region of the human GAD 65 protein.
SpecificityReacts with a 65kD protein, corresponding to human GAD (Glutamate Decarboxylase). Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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